Part:BBa_K260007

pMA-@CC2FosMB-05000bp

This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 05000 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.

The pMA-@CC2FosMB-05000bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260007|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 05000 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 5,000 bp exactly.

These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:

[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp [[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp [[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp [[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp [[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2544
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2550
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2544
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2544
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2544
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2559
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.