![](https://parts.igem.org/images/partbypart/icon_terminator.png)
Terminator
Part:BBa_K4800001
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-04)
rrnB T1 terminator
Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)
Result:
Adjusting the distance between FNR binding site and the -35 region of promoter vgb fine tunes the inhibitory effect of oxygen on the promoter.
Fig.9 Construction of the recombinant plasmid for pMTL-PvgbF7-bs2
MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 357
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 357
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 357
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 357
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |