Terminator

Part:BBa_K4800001

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-04)
Revision as of 11:38, 4 October 2023 by ZQM 0907 (Talk | contribs)


rrnB T1 terminator

Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)



Document

Result:

Adjusting the distance between FNR binding site and the -35 region of promoter vgb fine tunes the inhibitory effect of oxygen on the promoter.

Fig.9 Construction of the recombinant plasmid for pMTL-PvgbF7-bs2

MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 357
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None