Part:BBa_K4653001

shRNA(CHSIIIa)-1

To kill B. cinerea, we designed two pieces of shRNAs targeting the CHSIIIa gene of the pathogen, which is essential for its cell wall formation, based on RNAi technology.

Sequencing

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Biology

Chitin synthase (CHS) catalyzes the synthesis of chitin, a major structural component of fungal cell walls consisting of β-1, 4-linked N-acetylglucosamine polymers. CHS can be divided into seven classes, and ascomycetes usually have representatives of all seven classes, with each enzyme having a specific role. CHS (III, V, and VI) are specific to molds, suggesting that they may play an important role in hyphal growth. By silencing the important chitin synthase gene BcCHSIIIa, the formation of the cell wall of B. cinerea could be affected, thus killing pathogenic fungi.

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