DNA

Part:BBa_K4897002

Designed by: Ji Zhiyuan   Group: iGEM23_BS-United-China   (2023-10-03)
Revision as of 01:41, 4 October 2023 by Registry (Talk | contribs)

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BS DNA-322 (BS DNA 3.0) using in L-RCA for detecting P. acne

BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. Binding region is the key element in reacting with P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single strand DNA meanwhile the phi29 will generate double stranded DNA through amplification primers

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 161
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 161
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 161
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None