Coding

Part:BBa_K4593003:Design

Designed by: Zixuan Zhou   Group: iGEM23_BNDS-China   (2023-09-27)
Revision as of 15:03, 3 October 2023 by Jianfei Song (Talk | contribs) (→‎Design Notes)


LysGH15


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 1323
    Illegal AgeI site found at 793
    Illegal AgeI site found at 1021
    Illegal AgeI site found at 1192
    Illegal AgeI site found at 1305
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A

Source

staphylococcal phage GH15

References

Gu, J., Feng, Y., Feng, X., Sun, C., Lei, L., Ding, W., Niu, F., Jiao, L., Yang, M., Li, Y., Liu, X., Song, J., Cui, Z., Dong Soo Han, Du, C., Yang, Y., Liu, Z.-J., Liu, Z.-J., & Han, W. (2014). Structural and Biochemical Characterization Reveals LysGH15 as an Unprecedented “EF-Hand-Like” Calcium-Binding Phage Lysin. 10(5), e1004109–e1004109. https://doi.org/10.1371/journal.ppat.1004109