Coding

Part:BBa_K4839023

Designed by: Xuming Zeng   Group: iGEM23_SYSU-SLS-CHINA   (2023-10-03)
Revision as of 08:24, 3 October 2023 by Registry (Talk | contribs)

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TRE3GS promoter- Anti- IRF4 BioPROTAC

This component consists of promoter PGK, downstream rtTA, and promoter TRE3GS, downstream Anti-IRF4 BioPROTAC. rtTA acts as a trans-activator in the Tet-inducible transcription system (Tet-ON system) and binds to the exogenous Dox to activate pTRE, initiating downstream transcription, and the downstream Anti-IRF4 BioPROTAC encoded product recognizes and binds IRF4 and induces its ubiquitinated degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1258
    Illegal SpeI site found at 257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 257
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1258
    Illegal SpeI site found at 257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1258
    Illegal SpeI site found at 257
    Illegal NgoMIV site found at 2127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99
    Illegal BsaI.rc site found at 2508
    Illegal SapI.rc site found at 2522


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Categories
Parameters
None