Coding

Part:BBa_K4905005

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-07-23)
Revision as of 08:29, 3 October 2023 by Merel (Talk | contribs)

Leucine zipper Z2

Information

Basic-region Leucine zippers (bZIPs) are alpha-helical domains with a repeating unit “abcdefg”. In this unit, the positions “a” and “d” consist of a hydrophobic residue and the ”e” and “g” positions consist of charged residues. The zippers form an alpha-helix and their charged residues form ion pairs between helices, causing them to associate[1].

Individual bZIP proteins can form homodimers or heterodimers with other bZIP proteins with a slightly different sequence[1]. Not all bZIP proteins can dimerize with each other, some will only homodimerize with the same bZIP protein. Others will only heterodimerize with specific other bZIP proteins[2].

In cells, the main function of bZIPs is that they work as transcription factor, where the homodimer or heterodimer will form at the promotor regions of target genes[3]. This makes leucine zippers a large family of transcription factors, where each member has a preference for a specific DNA sequence[2].

This specific Leucine zipper was designed with inspiration from Gradišar et al.[4] where they used it, together with Elastin-Like Polypeptides (ELPs) to form a reversible, injectable hydrogel. Z2 is one of the two complementary Leucine zippers that they used in this research. They found that the thermosensitive response of ELPs together with the dimerization of Leucine zippers enables the formation of a thermosensitive hydrogel.

The TU-Eindhoven 2023 team used this part in composite part BBa_K4905006 for the formation of a hydrogel in E. coli. This composite part consists of ELPs together with two complementary Leucine zippers at their end. This part is one of these bZIP proteins.

Characterization

We ran an electrophoresis gel with the plasmid, digested with enzymes AcuI and BglI. The band of Z2 is shown in slot 5 in the gel. A 1 kb ladder (L) was used. The remaining slots were used for different parts. The bands formed as expected.

  1. A120: BseRI + BglI, with CIP
  2. Z1.1: Acul + BglI, without CIP
  3. A100: BseRI + BglI, with CIP
  4. Z1.2: Acul + BglI, without CIP
  5. Z2: Acul + BglI, without CIP

Figure 1 | Electrophoresis gel with leucine zipper Z2 in slot 5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Alber, T. (1992). Structure of the leucine zipper. Current Opinion in Genetics and Development, 2, 205–210.

[2] Hakoshima, T. (n.d.). Leucine Zippers. https://doi.org/10.1038/npg.els.0005049

[3] Seldeen, K. L., McDonald, C. B., Deegan, B. J., Bhat, V., & Farooq, A. (2010). Dissecting the Role of Leucine Zippers in the Binding of bZIP Domains of Jun Transcription Factor to DNA. Biochemical and Biophysical Research Communications, 394(4), 1030. https://doi.org/10.1016/J.BBRC.2010.03.116

[4] Gradišar, H., & Jerala, R. (2010). De novodesign of orthogonal peptide pairs forming parallel coiled-coil heterodimers. Journal of Peptide Science, 17(2), 100–106. https://doi.org/10.1002/psc.1331

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