DNA

Part:BBa_K4765112

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 14:36, 1 October 2023 by Zhenmaoye (Talk | contribs) (→‎Usage and Biology)


Twister P1 + T7_RBS + SAHS 33020 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in Secretory-abundant heat soluble protein 33020 (SAHS 33020).

Usage and Biology

We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant capability. And this composite part can cooperate with ribozyme+T7_RBS+mtSSB+stem-loop to increase the survival rate of E.coli under desiccation.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178


Reference

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Categories
//collections
Parameters
protein