DNA

Part:BBa_K4765102

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-09-30)
Revision as of 12:50, 1 October 2023 by Zhenmaoye (Talk | contribs)


Twister P1 + T7_RBS + intimin-Nb1 fusion + stem-loop

contributed by Fudan iGEM 2023

Usage and Biology

Similar to ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop, we replaced Nb2 with Nb1 in order to interact with ribozyme+strong RBS+intimin-Ag1 fusion+stem-loop to create a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305


[edit]
Categories
//collections/biofilm
Parameters
protein