Part:BBa_K4765121

ribozyme connected: galU + pgmA + mScarlet

Introduction

This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes ’’E. coli’’ galU on the upstream site and ’’E. coli’’ pgmA on the downstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in E.coli galUand E.coli pgmA. The following mScarlet is utilized to detection E. coli with adhesion ability.

Usage and Biology

We use this part to improve EPS production of ’’E. coli’’ and assessment of EPS’s adhesion ability.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 3285
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]