Part:BBa_K4765111

Twister P1 + T7_RBS + AnAFP + stem-loop

contributed by Fudan iGEM 2023

This composite part is composed of AnAFP and ribozyme-assisted polycistronic co-expression system：pRAP. AnAFP is an antifreeze protein derived from Ammopiptanthus nanus. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions ^[1].

Usage and Biology

The heterologously expressed codon-optimized AnAFP can endowing E.coli, with antifreeze capability.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 512

Reference

↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. ’‘Gene, 539’‘(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013

[1] Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. ’‘Gene, 539’‘(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013

[1]

Part:BBa_K4765111

Contents

Introduction

Usage and Biology

Characterization

Reference