DNA

Part:BBa_K4765119

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 12:46, 1 October 2023 by Zhenmaoye (Talk | contribs)


ribozyme test: constitutive expression

contributed by Fudan iGEM 2023

Usage and Biology

We designed an assessment setup to characterize the performance of pRAP. This setup incorporates Staygold, mScarlet and two adjustable-strength RBS elements. Initially, we employed the assessment setup with the same RBS to validate regular protein expression in pRAP.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1399
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690
    Illegal BsaI.rc site found at 710


[edit]
Categories
//collections
Parameters
None