Composite

Part:BBa_K4614105

Designed by: Zhang Xiyuan   Group: iGEM23_CAU-China   (2023-09-30)
Revision as of 14:14, 30 September 2023 by Registry (Talk | contribs)

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T7-RBS-Wza-SpyCatcher

T7-RBS-wza-SpyCatcheris composed of T7 promoter, RBS and T7 terminator from plasmid pET-30a(+), wza gene (BBa_K4614104), SpyCatcher gene (BBa_K4614103). This composite part is designed to use protein wza to display the protein SpyCatcher at a relatively fixed position on the surface of bacteria to give it the ability to cross-link at specific locations on the bacterial surface. Since there is a lac operator sequence behind the T7 promoter of plasmid pET-30a(+), the display effect of SpyCatcher can be artificially controlled by the time and concentration of IPTG addition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 704


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