Translational_Unit

Part:BBa_K216008

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)
Revision as of 14:25, 16 October 2009 by Registry (Talk | contribs)

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bacterial luciferase LuxAB of Xenorhabdus luminescens

This part includes the coding sequences of luxA and luxB of Xenorhabdus luminescens, with their native ribosome binding sites. The dimeric bacterial luciferase LuxAB produces light in the presence of oxygen, FMNH2, and a long chain aldehyde. The aldehyde can be provided by the luxCDE gene products, but if these are not present, n-decanal can be added to cultures.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1049


[edit]
Categories
//function/reporter/light
Parameters
None