Part:BBa_K4814003

Linkers can be categorized into three types based on their structures: flexible linkers, rigid linkers, and in vivo cleavable linkers. Flexible and rigid linkers serve the purpose of connecting functional domains, while in vivo cleavable linkers facilitate the release of free functional domains within the body. Apart from their linking function, linkers offer additional advantages in the production of fusion proteins, such as enhancing biological activity, increasing expression yield, and achieving favorable pharmacokinetic profiles.

In recent research by Meyers, W. M., et al (2023), the researcher used 3xGGGGs as a linker in their mammalian cell virus design. They used HEK embryonic kidney cells to test.

3xGGGGs is a commonly used flexible linker for fusing two proteins.

References:

Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039

Meyers, W. M., Hong, R. J., Sin, W. C., Kim, C. S., & Haas, K. (2023). A cell-based assay for rapid assessment of ACE2 catalytic function. Scientific Reports, 13, 14123. https://doi.org/10.1038/s41598-023-41389-7

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]