Coding

Part:BBa_K185049

Designed by: Alex Jiang   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-16)
Revision as of 09:42, 17 October 2009 by Nothingletitled (Talk | contribs)

Lon protease

The ATP-dependent Lon protease is a member of the AAA+ family of proteins (ATPases associated with various cellular activities), which is present in archaea, prokaryotes and eukaryotic mitochondria, peroxisomes and plastids. Lon has multiple cellular functions such as degrading abnormal polypeptides, as well as certain regulatory proteins and metabolic enzymes, acting as a chaperone and binding to nucleic acids. In our project, since the Lon protease can hydrolysis RelB protein efficiently, we use it to balance the concentration of relE and relB inside E.coli. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 619
    Illegal AgeI site found at 1795
    Illegal AgeI site found at 1879
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2110
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None