Part:BBa_K4642021
Gen4 373-21-24 Double AND-Gate Toehold Switch
Toehold switches are a class of de-novo-designed riboregulators that enable posttranscriptional activation of protein translation through mechanisms employed in artificial systems rather than natural ones. Unlike conventional riboregulators, the switches take advantage of toehold-mediated linear-linear interactions developed in vitro to initiate RNA-RNA strand displacement interactions. Furthermore, they rely on sequestration of the region around the start codon to repress protein translation, eschewing any base pairing to the RBS or start codon itself to regulate translation.
As a result, toehold switches can be designed to activate protein translation in response to a trigger RNA with an arbitrary sequence, enabling substantial improvements in component orthogonality. The absence of binding to the RBS and use of thermodynamically favourable linear-linear interactions also enables facile tuning of translational efficiency via RBS engineering. Consequently, these systems routinely enable modulation of protein expression over two orders of magnitude.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |