Coding

Part:BBa_K4886000:Design

Designed by: Ruyi Shi   Group: iGEM23_Nanjing-BioX   (2023-07-03)
Revision as of 07:43, 3 July 2023 by Registry (Talk | contribs)

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Phosphoketolase(FXpk) from Bifidobacterium adolescentis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1675
    Illegal PstI site found at 299
    Illegal PstI site found at 1970
    Illegal PstI site found at 2450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1675
    Illegal PstI site found at 299
    Illegal PstI site found at 1970
    Illegal PstI site found at 2450
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1675
    Illegal PstI site found at 299
    Illegal PstI site found at 1970
    Illegal PstI site found at 2450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1675
    Illegal PstI site found at 299
    Illegal PstI site found at 1970
    Illegal PstI site found at 2450
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a key enzyme in the NOG pathway.


Source

The gene is derived from Clostridium acetobutylicum ATCC824.

References