Part:BBa_K4886004
Pfba-FXpk(BD)
It is a part that is responsible for expressing F/Xpk from Clostridium tyrobutyricum. with fba promotor. X-FXpk(BD) vector (7670 bp) was obtained by using the constructed recombinant plasmid Pthl-FXpk(BD) as template and X-PN-F and X-PN-R as primers. Using the genome of Clostridium butyricum as template, P-Ptkt-F and P-Ptkt-R as primers, Ptkt gene fragment (300bp) was amplified. Gibson assembly method was used to connect the Ptkt fragment to the X-FXpk(BD) linearized vector. Colony PCR (1396 bp) was performed on the transformed colonies with primers P-Ptkt-F and CX. The positive colonies with correct colony PCR were transferred, plasmid was extracted, and the recombinant plasmid was obtained after sequencing verification.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1689
Illegal XbaI site found at 632 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1689
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1689
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1689
Illegal XbaI site found at 632 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1689
Illegal XbaI site found at 632 - 1000COMPATIBLE WITH RFC[1000]
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