Coding

Part:BBa_K4824000:Experience

Designed by: Jing Shi   Group: iGEM23_Bioplus-Shanghai   (2023-08-22)
Revision as of 06:35, 25 September 2023 by Ljj (Talk | contribs)


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Applications of BBa_K4824000

Introduction

Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.

User Reviews

UNIQf1cf68f03d7d042a-partinfo-00000000-QINU UNIQf1cf68f03d7d042a-partinfo-00000001-QINU