Plasmid_Backbone

Part:BBa_K4727003:Design

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-07-24)
Revision as of 16:18, 24 July 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


M13 standard phagemid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 623
    Illegal suffix found at 645
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 623
    Illegal SpeI site found at 646
    Illegal PstI site found at 660
    Illegal NotI site found at 629
    Illegal NotI site found at 653
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 623
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 623
    Illegal suffix found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 623
    Plasmid lacks a suffix.
    Illegal XbaI site found at 638
    Illegal SpeI site found at 646
    Illegal PstI site found at 660
    Illegal NgoMIV site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2026
    Illegal SapI site found at 943


Design Notes

Starting from the commercialy available pTZ19R (ThermoFisher scientific) a 45bp long region containing 3 prohibited restriction sites (EcoRI, XbaI and PstI) was deleted. Through PCR mutagenesis the BioBrick RFC[10] prefic and suffix were added.


Source

Deirived from pTZ19R by ThermoFischer scientific, a pUC19 plasmid containig the phage f1 intergenic region anche the T7 promoter

References