Coding

Part:BBa_K4606005

Designed by: Litong, Wu   Group: iGEM23_Nanjing-NFLS   (2023-09-14)
Revision as of 02:10, 19 September 2023 by Zoutong (Talk | contribs)


miR-3074-5p coding sequence

Description

Here we construct the sequences that encoding miR-3074-5p. The gene sequence encoding miRNA is first transcribed into pri-miRNA, which is cleaved and modified by ribonuclease to become the final miRNA. MiRNAs are a class of endogenous non-coding single-stranded small RNAs with a length of approximately 20–22 nucleotides and their expression changes are related to many human diseases.

After inserting this sequence into the vector and expressing it in cells, we could test whether the miR-3074-5p was able to target the SMAD4 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None