Coding

Part:BBa_K4606003:Design

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)
Revision as of 01:59, 23 September 2023 by Alex2006 (Talk | contribs) (→‎Design Notes)


SMAD4(wild type) SMAD4 is important for cartilage homeostasis. The inhibition of SMAD4 can induce chondrocyte apoptosis and aggravate osteoarthritis progression. Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in the course of osteoarthritis. We constructed a portion of the gene encoding SMAD4. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment. This fusion expression does nearly no harm to the normal physiological state of firely luciferase.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in osteoarthritis. Here we designed the base sequence of the base segment binding to 3074 as well as the segment of its upstream and downstream

Source

AAA

References