Translational_Unit

Part:BBa_K4586026

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 14:45, 20 September 2023 by Mohamed-Ab0elghare (Talk | contribs) (→‎Usage)


Loading system (CD63-L7Ae)


This composite part coding for our loading system that is composed of two components: L7Ae, which is a RNA binding protein that have an affinity to C\D box or kink turn coupled present within the 3` end of our cargo. This RNA binding protein is conjugated to the C terminal end of CD63, which is highly expressed trans membrane protein on exosomes surface.

Usage

This part is implemented in our design to mediate the loading of our therapeutic agent into the engineered exosomes thus by labeling our cargo with a C\D box in the 3` prime end in order to bind to the L7Ae and load the cargo in form of RNA selectively into the exosomes that targets the autoreactive B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None