Coding

Part:BBa_K4586015

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 12:56, 21 September 2023 by Mahmoud sayed (Talk | contribs) (→‎Usage)


L7Ae

Part Description

L7Ae is a 50S ribosomal protein that is considered an RNA-binding protein. It has the ability to recognize its K-turn motif or C/D box in ribosomal RNA and can be used to build more complicated genetic circuits that are regulated at both the translational and transcriptional levels as well as as an adapter for RNA binding in the extracellular vesicles.

Usage

Our team uses this part in loading our cargo into the exosomes. By binding to the C/D boxes to load a specific RNA into the vesicles, we fused it to RNA-motifs, which are bound specifically by RNA-binding proteins. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None