Regulatory
Ptet*

Part:BBa_K4579000

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-09-17)
Revision as of 03:21, 22 September 2023 by Alexakmorton (Talk | contribs)


PTet* promoter + RBS

This is an inducible promoter part compatible with Golden Gate Assembly. This part can undergo BsaI digestion to produce a linear part with overhangs consistent with the Bee Toolkit syntax (Leonard et al., 2018). ontains tetO operators that are repressed in the presence of tetR repressor protein. tetR can be removed from the promoter in the presence of anhydrotetracycline (aTc), allowing for the activation of transcription in cells containing both pTet and tetR. In strains only containing pTet, this promoter acts as in a functionally constitutive manner.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None