Device

Part:BBa_K283002

Designed by: Shi Lei, Nelson Chu, W Ling   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 07:21, 13 October 2009 by Registry (Talk | contribs)

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Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)

This is a device that enable the control of chez(histag) using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1782
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/device/uncategorized
//function/motility
Parameters
biology
controlaTc, tetracyline, IPTG, LacI
directionForward
negative_regulators2
positive_regulators
proteinschez ,TetR
tagLVA, 6-Histidine