Coding
Part:BBa_K4891013:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
pta
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.
Source
Recombinant plasmids pTargetF-sgRNAaroK/aroL/ptsH/ptsI initiate with pTargetF, which stores in our lab.