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Other
Part:BBa_K4891012:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
poxB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 651
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 651
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 651
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 651
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.
Source
Recombinant plasmids pTargetF-sgRNAaroK/aroL/ptsH/ptsI initiate with pTargetF, which stores in our lab.