![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4891009:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
adhE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 933
Design Notes
These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.
Source
AroK and AroL (encoding shikimic acid kinase), PtsH and PtsI (encoding phosphotransferase system) are obtained from the genome of E. coli MG1655.