Composite

Part:BBa_K4719018

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-06)
Revision as of 13:49, 6 September 2023 by Registry (Talk | contribs)

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pKARA_RT3 styrene monooxigenase for indigo synthesis in K. xylinus

To dye bacterial cellulose in situ we used flavin dependent monooxygenase pKARA_RT3 to achieve colorful bacterial cellulose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1226
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1226
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1226
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1226
    Illegal SpeI site found at 37
    Illegal NgoMIV site found at 92
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 503
    Illegal AgeI site found at 689
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None