Coding

Part:BBa_K4891002:Design

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)
Revision as of 06:21, 27 June 2023 by Registry (Talk | contribs)

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aroD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 21
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 208


Design Notes

For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.


Source

There are four genes for converting PEP and E4P into shikimate, including aroG (encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase), aroB (encoding 3-dehydroquinate synthase), aroD (encoding 3-dehydroquinate dehydratase), and aroE (encoding shikimate dehydrogenase), and these genes originate from Escherichia coli.

References