Composite

Part:BBa_K4656012

Designed by: Shuyang Zhu   Group: iGEM23_NMU-China   (2023-09-13)
Revision as of 12:58, 17 September 2023 by Juejue (Talk | contribs)


pT7-mleR-p_mleS-mazF

MleS placed downstream of the T7 promotor is a malate-binding transcriptional activator belonging to l. lactis, which has been characterized by iGEM22_GEMS_Taiwan in 2022. P_MleS is taken from the putative promoter region of mleS by iGEM22_GEMS_Taiwan, this is, it is a binding site of MleS.

Usage and Biology

In our circuit, the Mles can tightly bind to P_MleS in the presence of malate, which can change the conformation of Mles, thereby activating P_MleS and the expression of downstream mazF. In this design, the addition of malate can inhibit the growth of engineered E. coli and induce apoptosis.

Experimental results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 68
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None