Plasmid

Part:BBa_K4605010

Designed by: Jinran Liu   Group: iGEM23_Nanjing-China   (2023-09-13)
Revision as of 05:32, 16 September 2023 by BetaGlobin (Talk | contribs)


Used to produce indigo in Corynebacterium glutamicum

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Sequence and Features 2000marker.png


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3789
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 25
    Illegal XbaI site found at 9532
    Illegal SpeI site found at 7594
    Illegal SpeI site found at 7973
    Illegal PstI site found at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3789
    Illegal EcoRI site found at 3940
    Illegal SpeI site found at 7594
    Illegal SpeI site found at 7973
    Illegal PstI site found at 13
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3789
    Illegal EcoRI site found at 3940
    Illegal BglII site found at 3932
    Illegal BamHI site found at 31
    Illegal XhoI site found at 3085
    Illegal XhoI site found at 3784
    Illegal XhoI site found at 6063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3789
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 25
    Illegal XbaI site found at 9532
    Illegal SpeI site found at 7594
    Illegal SpeI site found at 7973
    Illegal PstI site found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3789
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 25
    Illegal XbaI site found at 9532
    Illegal SpeI site found at 7594
    Illegal SpeI site found at 7973
    Illegal PstI site found at 13
    Illegal NgoMIV site found at 331
    Illegal NgoMIV site found at 738
    Illegal NgoMIV site found at 9257
    Illegal NgoMIV site found at 10601
    Illegal AgeI site found at 1996
    Illegal AgeI site found at 2049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 274
    Illegal BsaI site found at 1585
    Illegal BsaI site found at 2299
    Illegal BsaI site found at 4139
    Illegal BsaI.rc site found at 403
    Illegal BsaI.rc site found at 3589
    Illegal SapI site found at 5029
    Illegal SapI.rc site found at 1837


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