Regulatory

Part:BBa_K203112:Design

Designed by: Lars Velten and Simon Haas   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 14:49, 16 October 2009 by Larsplus (Talk | contribs) (→‎Design Notes)

JeT; constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was adapted from [1] to include a HindIII site between core and proximal promoter (after base pair 66). Sequence was then synthesized by Assembly PCR [2] using Oligos designed using [3]:

JeT 01 GTACCAATTGGTGAGAATTCACTAGTGGGCGGAGTTA JeT 02 CGCTGATTGGCTCCGCCCTAACTCCGCCCACTAGTGA JeT 03 AGGGCGGAGCCAATCAGCGTGCGCCGTTCCGAAAGTT JeT 04 CGCCCAGCCATAAAAGGCAACTTTCGGAACGGCGCAC JeT 05 TGCCTTTTATGGCTGGGCGGAAGCTTAGAATGGGCGG JeT 06 TATAATCATCGGCGTTCACCGCCCATTCTAAGCTTCC JeT 07 GTGAACGCCGATGATTATATAAGGACGCGCCGGGTGT JeT 08 GACGGAACTAGCTGTGCCACACCCGGCGCGTCCTTAT JeT 09 TGGCACAGCTAGTTCCGTCGCAGCCGGGATTTGGGTCG JeT 10 CTAGCACAAACAAGAACCGCGACCCAAATCCCGGCTGC JeT 11 CGGTTCTTGTTTGTGCTAGCCTGCAGGTCAGAGATTGA JeT 12 AGTCTGATCAATCTCTGACCTGCAGG

Source

To be up dated

References