![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4891001:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
aroB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 906
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.
Source
There are four genes for converting PEP and E4P into shikimate, including aroG (encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase), aroB (encoding 3-dehydroquinate synthase), aroD (encoding 3-dehydroquinate dehydratase), and aroE (encoding shikimate dehydrogenase), and these genes originate from Escherichia coli.