Regulatory

Part:BBa_K4656005

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-08)
Revision as of 11:28, 10 August 2023 by GuXinji (Talk | contribs)


PpchA

PpchA, the promoter of pchA, is present in the butyrate sensor derived from EHEC strain O157:H7, which was discovered by Nakanishi et al. (2009).[1] In the butyrate sensor, PpchA binds to a complex formed by leucine response regulatory protein (Lrp) and butyrate to activate the transcription of pchA regulators.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75


Reference

[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.

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