Coding

Part:BBa_K4656003

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-07)
Revision as of 15:31, 7 September 2023 by Juejue (Talk | contribs)


Lrp

The Lrp is a gene encoding leucine reactive protein, which is expressed by E. coli itself. It mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons, and Lrp mediates this effect for at least some of these operons. For example, it is a regulator of the branched-chain amino acid transport genes.

In our project, Lrp dimerizes with butyrate, binds to pPchA promoter, and further produces pchA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None