Coding

Part:BBa_K4865000

Designed by: Jing Shi   Group: iGEM23_OTIA-Hangzhou   (2023-08-22)
Revision as of 06:29, 22 August 2023 by Registry (Talk | contribs)

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EGF without start condon

This part generates epidermal growth factor (EGF). And it is a small protein involved in epidermal cell growth, oncogenesis, and wound healing. The original signal sequence was replaced with pelB signal sequence to send the protein to periplasmic space on and then be cleaved the 5' end this also allows for potential increased stability of the folded protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 134


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Categories
Parameters
None