Coding

Part:BBa_K4593001

Designed by: Jianfei Song   Group: iGEM23_BNDS-China   (2023-08-21)
Revision as of 21:15, 21 August 2023 by Jianfei Song (Talk | contribs)


Spn1s_LysRZ


Usage and Biology

Spn1s_LysRZ is an protein complex that is consists of holin, endolysin and two Rz/Rz1-like protein that act together to exhibit high lytic activity against E. coli and Salmonella Typhimurium.


Team:BNDS-China 2023

Our project intends to develop an in-vivo elimination capsule targeting S. aureus. Spn1s_LysRZ is used to lyse E. coli and release the S. aureus targeting endolysin it expressed.

To characterize the lytic efficiency of Spn1s_LysRZ, we designed plasmid Spn1s_LysRZ-pET28a (assembled by Genscript) (Fig. 1), which allows Spn1s_LysRZ to be expressed under the presence of IPTG.


Fig. 1 The plasmid map of Spn1s_LysRZ-pET28a




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 890
    Illegal AgeI site found at 1028
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None