RNA

Part:BBa_K4887002:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-BioX   (2023-08-14)
Revision as of 09:42, 17 August 2023 by Htclamm (Talk | contribs)


sgRNA (IbGBSSI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sgRNA is a synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding/binding ability for Cas9 nuclease. This synthetic fusion does not exist in nature and is also commonly referred to as an sgRNA.

Source

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References