Coding

Part:BBa_K4656003:Design

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-07)
Revision as of 01:05, 9 August 2023 by GuXinji (Talk | contribs) (→‎References)


TPH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 898
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 240


Design Notes

LRP, as a component of the natural FIM system of E. coli, is an fimS regulator. Both LRP and IHF(Integrated Host factor) may act to regulate the actual inversion events through an involved DNA bending protein complex. In addition, there is the OFF-to-ON inhibitory protein papB and many other hypothesized and known regulators that directly act on LRP. During the course of the project, the FIM system can be considered. (Additional background on FIM systems can be found in the references [1])

Source

It is from the Escherichia coli K-12 Lrp gene for leucine responsive regulatory protein. Organism: Escherichia coli K-12

References

1. Schwan WR. Regulation of fim genes in uropathogenic Escherichia coli. World J Clin Infect Dis 2011; 1(1): 1725.