Regulatory

Part:BBa_K4656004:Design

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-08)
Revision as of 11:29, 10 August 2023 by GuXinji (Talk | contribs)


PLEE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is better to select the plasmids constructed with different PLEE1 fragments amplified by PCR to select the one with the best expression.


Source

The PLEE1 is from its organism, the Escherichia coli strain O157:H7. Organism: Escherichia coli O157:H7 str. TW14359

References