![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K228866
Designed by: Lin Min Group: iGEM09_PKU_Beijing (2009-09-22)
AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1274
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2007
Illegal BamHI site found at 1214 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1839
Illegal AgeI site found at 1320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1323
[edit]
Categories
Parameters
n/a | AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation) |