Coding

Part:BBa_K4905001

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-06-13)
Revision as of 17:11, 23 July 2023 by Merel (Talk | contribs)


Elastin-Like polypeptide (VPGIG)[60]-(VPGAG)3(VPGGG)2[12]

The TU-Eindhoven 2023 team used this part for the formation of a hydrogel in e.coli. The repeating sequence of this part is (VPGIG)60 and (VPGIG)60 which creates a hydrophilic and a hydrophobic part. It has been codon optimized for expression in e.coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1890
    Illegal XbaI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1890
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1890
    Illegal XhoI site found at 1907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1890
    Illegal XbaI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1890
    Illegal XbaI site found at 7
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 334
    Illegal NgoMIV site found at 514
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain
Parameters
None