Part:BBa_K4414005

GFP

Usage and Biology

There is an extensive use of fluorescent bioimaging in the fields of biochemistry, biotechnology, cell and developmental biology. In comparison with other fluorescent tags, GFP-like proteins have the advantage of forming internal chromophore without requiring accessory cofactors, enzymes or substrates other than molecular oxygen, making possible chromophore formation in live organisms, tissues and cells(Stepanenko et al., 2008;Cubitt et al., 1995). We obtained this DNA by DNA synthesis and used it in level 1 biological laboratory.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional characterization

We connect GFP as a reporter gene to other sequences encoding proteins, as shown in the figure is the fluorescence image of GFP.

Figure 1.The picture of fluorescence diagram

Reference

1.Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A., & Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends in Biochemical Sciences, 20(11), 448–455. https://doi.org/10.1016/s0968-0004(00)89099-4.

2.Stepanenko, O. V., Verkhusha, V. V., Kuznetsova, I. M., Uversky, V. N., & Turoverov, K. K. (2008). Fluorescent Proteins as Biomarkers and Biosensors: Throwing Color Lights on Molecular and Cellular Processes. Current Protein & Peptide Science, 9(4), 338–369.