Generator

Part:BBa_K4475003

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-07-18)
Revision as of 01:06, 14 October 2022 by Andrew Taylor (Talk | contribs)


CTB1 Constitutive Strong Protein Generator Yeast

Construct designed for strong expression of the fungal enzyme CTB1 in saccharomyces cerevisiae, utilizing the Yeast GPD (TDH3) Promoter and CYC100 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4213
    Illegal BamHI site found at 4122
    Illegal BamHI site found at 6295
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6802
    Illegal AgeI site found at 4876
  • 1000
    COMPATIBLE WITH RFC[1000]




[edit]
Categories
//regulation/constitutive
regulator
Parameters
device_type
efficiency
negative_regulators
positive_regulators