Plasmid_Backbone

Part:BBa_K187001:Design

Designed by: Team BioBytes, University of Alberta   Group: iGEM09_Alberta   (2009-10-15)
Revision as of 03:44, 16 October 2009 by Registry (Talk | contribs)

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pBA Universal Plasmid for BioBytes Gene Assembly System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 750
    Illegal XbaI site found at 792
    Illegal PstI site found at 837
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 750
    Illegal PstI site found at 837
    Illegal NotI site found at 756
    Illegal NotI site found at 861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 750
    Illegal XbaI site found at 792
    Illegal PstI site found at 837
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 750
    Illegal XbaI site found at 792
    Illegal PstI site found at 837
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1727
    Illegal SapI.rc site found at 846


Design Notes

Using these plasmids as well as universal primer sequences for the PCR extraction of bricks from the plasmids anyone will be able to use this system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of the BioBytes system


Source

Derivative of pUC19

References