Composite
Part:BBa_K4390092
Designed by: Zhongyi Liang Group: iGEM22_Edinburgh-UHAS_Ghana (2022-09-14)
His-tagged WT RolA
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
Usage and Biology
The wild-type RolA with 6His-tag for Nickel affinity column purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 169
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 161
Reference
[edit]
Categories
Parameters
None |