Generator

Part:BBa_K4167660

Designed by: Lin Wang   Group: iGEM22_ICJFLS   (2022-10-13)
Revision as of 19:50, 13 October 2022 by Registry (Talk | contribs)

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LacZ generator driven by Plac

This is a β-galactosidase protein generator with strong RBS, driven by Plac promoter. IPTG can bind to the operator site of the promoter to induce β-galactosidase expression. With the different concentration of IPTG, it can express β-galactosidase at different level. With the detection of p-Nitrophenol, the activity of β-galactosidase can be measured.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None